Journal: The Journal of Biological Chemistry

Article Title: Human CD300C Delivers an Fc Receptor-?-dependent Activating Signal in Mast Cells and Monocytes and Differs from CD300A in Ligand Recognition *

doi: 10.1074/jbc.M112.434746

Figure Lengend Snippet: Cell surface expression of CD300A and CD300C in human hematopoietic cells. A, analysis of CD300A or CD300C expression in hematopoietic cells derived from human PB. FSChighSSChigh populations in neutrophils or eosinophils or FSClow/intSSClow/int populations in basophils, T cells, B cells, monocytes, NK cells, or PDCs were gated. The cells were stained with FITC-conjugated anti-CD3, CD19, CD56, BDCA-2, CD123, or CD16 mAb or allophycocyanin-conjugated anti-CD14 mAb and analyzed for CD300A or CD300C expression. B, human PB-derived Mφ-1 or Mφ-2 were stained with R-PE-conjugated anti-CD11b, CD14, or HLR-DR mAb or FITC-conjugated CD11c mAb and were analyzed for CD300A or CD300C expression. C, human monocyte-derived DCs were cultured for 24 h in the presence or absence of 1 ng/ml LPS or 10 ng/ml TNFα. The cells were stained with R-PE-conjugated anti-CD80, CD83, or CD86 mAb and were analyzed for CD300A or CD300C expression. D, human PB-derived mast cells were analyzed for CD300A or CD300C expression. The results of control staining are shown as filled histograms. All of the data are representative of three independent experiments.

Article Snippet: R-PE-conjugated anti-human blood dendritic cell antigen-2 mAb and FITC-conjugated CD16 or CD123 mAb were from Miltenyi Biotech.

Techniques: Expressing, Derivative Assay, Staining, Cell Culture, Control